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Hi,
We previously had the Xe lamps and changed to the SSI illumination on
our Deltavision Core. The exposure times were reduced by a factor of 5
to 10. The SSI is actually made by Lumencor (no commercial interest).
From my experience it works really well without the downsides of a lamp
of heat generation or having to align and change bulbs.
If you do decide to use LEDs, I strongly suggest still using bandpass
excitation filter (as suggested previously) because with some LEDs there
upto 5% light emission at wavelengths upto 50 nm higher than the peak
which will generally add a lot of background to your images. If you have
a spectrometer available I suggest scanning the emission to get the real
emission spectrum of the LED.


Regards,
Justin.

-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]]
On Behalf Of Julio Vazquez
Sent: Friday, 24 February 2012 4:14 AM
To: [log in to unmask]
Subject: Re: LEDs

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Hello all, 

Applied Precision just told me that the InsightSSI illuminator is
actually not LED based, so please ignore my previous posting, as far as
LEDs are concerned.

Julio.

==


On Feb 23, 2012, at 2:06 AM, jacques wrote:

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> To join, leave or search the confocal microscopy listserv, go to:
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> *****
> 
> Thanks for your help and comments !
> 
> Jacques