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Esteban,

I am curious what kind of sample you expose for 30-60 seconds? Won't
background fluorescence kill you?

Paul Herzmark
Department of Molecular and Cell Biology
University of California, Berkeley


On Sat, May 12, 2012 at 2:28 PM, G. Esteban Fernandez <
[log in to unmask]> wrote:

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>
> Hi all,
>
> 1)  I'm curious how much time, roughly, it takes for a Peltier-cooled
> CCD camera to reach the lowest temperature.  Are we talking a few
> minutes or closer to an hour or more?  I have several brands of cooled
> CCDs of different vintages and I'm just looking for a "rule of thumb",
> if possible.
>
> 2) I do plan to test my cameras myself:  I was thinking of imaging a
> timelapse of a dim & stable specimen (requiring something like 30-60
> sec. exposure) after turning cooling on to see at what time noise
> reaches a minimum.  Any suggestions for other ways to test this are
> welcome.
>
> Thanks,
> Esteban
>