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Date: | Wed, 12 Sep 2012 02:35:40 -0500 |
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Dear Vitaly,
Frankly, there is no really specific nuclear membrane dye. I remember a
paper from Zal et al., (Traffic 2006; 7: 1607–1613), which says the dye
FM4-64 is specific for the nuclear membrane, though in our tests the results
were inconsistent and it also stained other membrane entities.
I would suggest to use anti-NPC antibodies - these should specifically stain
the nuclear membrane (in interphase cells) - famous in the field is mAb414.
hope this helps,
Josef
On Tue, 11 Sep 2012 14:57:00 -0700, Vitaly Boyko <[log in to unmask]> wrote:
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>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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>
>Dear All,
>
>one of our users is looking for a relatively abundant marker that is
homogeneously distributed over the nuclear membrane in cultured human cells
(it could be a well characterized Ab against the nuclear receptor, or simply
specific (nuclear) membrane stain).
>
>Many thanks in advance,
>
>Vitaly
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