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Hi Mike,

I like using Carolina Observation Gel (http://www.carolina.com/product/life+science/microscope+slides/protist+slides/carolina+observation+gel.do, no commercial interest). 

It is mouldable and non-toxic polydimethylsiloxane, which can be made into custom gaskets to seal slides (I cut them to shape with a needle). It allows gas exchange, but minimises evaporation.

Best wishes,
George

******************************
Dr. George Littlejohn
School of Biosciences,
University of Exeter,
Halpin Laboratory,
Geoffrey Pope Building,
Stocker Road,
EX4 4QD, UK
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      +44(0)1392 269297 (Office)
Fax:  +44(0)1392 263434
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On 18 Sep 2012, at 21:44, "Mike Tighe" <[log in to unmask]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
> 
> Does anybody know of a non toxic seal for coverslips? I am looking at live-dead stained bacteria (hoping to see them die) but I think that my agarose is shinking due to evaporation over time. I am worried that nail polish or vacuum grease will affect my results.
> 
> 
> 
> As usual, thanks for any help!
> 
> Mike