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I used to work for Aperio (nearly 1000 of the Digital Pathology
Association's estimated 1500 - 2000 installations world wide), but was laid
off in Dec 2012 as part of their on going merger with Leica Biosystems.
Given the nature of slide scanning these systems are great for large areas
but not for time lapse and not for live or even unfixed specimens.
When it comes to bacteria, you also need to be careful to do a live
evaluation of the system on offer - can you see what you need to see in the
images it produces. I never tried to scan a bacterial smear on an Aperio
ScanScope, but I was asked to scan some slides of gut tissue stained for H.
pilori, and even at 40x (the maximum resolution of the system being
evaluated) the details that the pathologists were looking for were not
there - this was no surprise though because they could not have seen those
details on a standard microscope even with 40x oil. By contrast, I always
had very good results scanning CytoSpin slides.
My advise is to scan _YOUR_ slides on every scanner you are considering and
pick the one that gives the best image.
Feel free to contact me through this list, through my personal email (
[log in to unmask]) or through my new consulting company (Image Incyte, LLC)
at [log in to unmask] for further discussion.
Chris Tully
Microscopy and Image Analysis Expert
[log in to unmask]
240-475-9753 (c)
[image: View my profile on LinkedIn]<http://www.linkedin.com/in/christully/>
On Fri, Mar 8, 2013 at 12:07 PM, Julio Vazquez <[log in to unmask]> wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> You may want to have a look at some of the digital pathology systems out
> there. We have one from an Austrain company (Tissuegnostics; Google them if
> you want to see their products), but many other vendors (Zeiss, Leica,
> etc..) offer similar products. The system is designed to scan slides (our
> stage can take eight, but there are automated systems for high throughput).
> System will autofocus, find objects (assuming good samples) and image them.
> Typicaly, these are used to scan tissue sections, but we have done smears,
> TMA slides, etc.... System cam be used in fluorescence or brightfield
> mode. Some systems are designed for routine scanning and digitization of
> slides; ours is more flexible in teh sense we can use any fluorescence
> filter, objective, etc, to suit our needs. This company has some videos on
> thier web site. Most of this stuff can be done with a standard Research
> microscope, using laser autofocus, image-based autofocus, or a combination
> of both, and the multi-location/tiling capabilities of most current
> acquisition software. Digital Pathology systems are just optimized for this
> kind of stuff. Maybe you can get a couple of vendors to run a demo for you.
> --
> Julio Vazquez
> Fred Hutchinson Cancer Research Center
> Seattle, WA 98109-1024
>
>
> http://www.fhcrc.org
>
> ==
>
>
> On Mar 7, 2013, at 10:53 PM, Pascal Weber wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > My problem is:
> > The diagnostic Turberculosis. For this, the system must be able to fully
> motorized
> > and several blades without human intervention.
> > For this I have four well slide where I do 40 fields at 40X dry,
> automatically
> > detect bacteria, transferring data in Excel and tell me if the blade is
> positive or
> > negative. I ran in a first time focus and retain, or regain, when the
> microscope
> > slide changes.
> > Thank you very much for these first answers.
>
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