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Gabriel,
I should stress that my toluene soaking suggestion has never been tried here in house and was only suggested as a last ditch effort to save a lens in a dire situation like the one you described. The other suggestions made already seem much "milder" and should probably be tried before the method I mentioned. Try at your own discretion!... And do let us know how it all goes afterwards.
Cheers,
John Oreopoulos
On 2013-04-27, at 7:21 AM, phil laissue wrote:
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>
> The tiniest bit of (warm) distilled water in a small beaker, just
> enough to touch the front lens, may do the trick. And Zeiss also had a
> crystallising immersion oil several years ago, without green crystals
> though.
> _____________________________________
> Philippe Laissue, PhD, Bioimaging Manager
> School of Biological Sciences, Room 4.17
> University of Essex, Colchester CO4 3SQ, UK
> (0044) 01206 872246 / (0044) 07842 676 456
> [log in to unmask]
> privatewww.essex.ac.uk/~plaissue
>
>
> On Sat, Apr 27, 2013 at 11:23 AM, Matthew Nicholas
> <[log in to unmask]> wrote:
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>>
>> I've never dealt with this problem before, so this may be off the mark, but
>> what about using more of the oil itself to dissolve the crystals?
>>
>> Good luck!
>> Matt
>>
>> On 4/26/13 10:47 PM, Tina Carvalho wrote:
>>>
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>>>
>>> True, that. I had green crystals form in Leica oil as well! I can't
>>> remember for sure, but I think we heated it to dissolve them, or at least
>>> mobilize the oil enough that could be gently wiped off before trying any
>>> cleaner.
>>>
>>> Good luck and aloha,
>>> Tina
>>>
>>>>
>>>> I should add that the oil with the crystals was from Leica.
>>>> Joel
>>>>
>>>>
>>>> On Fri, Apr 26, 2013 at 8:30 PM, JOEL B. SHEFFIELD <[log in to unmask]>
>>>> wrote:
>>>>
>>>>> Wow! Toluene? We have had the experience of immersion oil crystalizing
>>>>> in the vial. Heating it slightly made the crystals dissolve. Perhaps a
>>>>> warm bath would be helpful in this case, or just warming the lens to 38
>>>>> or
>>>>> so..
>>>>>
>>>>> Joel
>>>>>
>>>>> On Fri, Apr 26, 2013 at 6:57 PM, John Oreopoulos <
>>>>> [log in to unmask]> wrote:
>>>>>
>>>>>> *****
>>>>>> To join, leave or search the confocal microscopy listserv, go to:
>>>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>>>>> *****
>>>>>>
>>>>>>> Hey Gabriel,
>>>>>>>
>>>>>>> I consulted with one of the optics experts over here and he recommends
>>>>>>
>>>>>> the following:
>>>>>>>
>>>>>>>
>>>>>>> Fill a beaker with toluene and create a rig above the beaker that will
>>>>>>
>>>>>> allow you to hold the tip of the objective with the oily crystals
>>>>>> submerged
>>>>>> in the toluene. Leave it there for a week, take it out and then
>>>>>> re-examine
>>>>>> the lens. Hopefully you can use standard cleaning procedures after that
>>>>>> for
>>>>>> any residual muck. Best of luck!
>>>>>>>
>>>>>>>
>>>>>>> John Oreopoulos
>>>>>>> Staff Scientist
>>>>>>> Spectral Applied Research
>>>>>>> Richmond Hill, Ontario
>>>>>>> Canada
>>>>>>> www.spectral.ca
>>>>>>>
>>>>>>> On 2013-04-26, at 2:03 PM, Gabriel Lapointe <
>>>>>>
>>>>>> [log in to unmask]> wrote:
>>>>>>>
>>>>>>>
>>>>>>>> *****
>>>>>>>> To join, leave or search the confocal microscopy listserv, go to:
>>>>>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>>>>>>> *****
>>>>>>>>
>>>>>>>> I have a problem with a dirty objective installed on a straight Leica
>>>>>>>> microscope. The lens is covered in what looks like oil mixed with
>>>>>>>> solid
>>>>>>>> dark green particles and the metal edge with green oily crystal. That
>>>>>>>> system hasn't been used for a while so I’m inclined to think that
>>>>>>
>>>>>> immersion
>>>>>>>>
>>>>>>>> oil was left on the objective for more than a year.
>>>>>>>>
>>>>>>>> Have you ever seen anything like that and do you have any suggestion
>>>>>>
>>>>>> as to
>>>>>>>>
>>>>>>>> how I should start the cleaning? I’m afraid to use my standard
>>>>>>>> cleaning
>>>>>>>> protocol as the particles inside the oil could scratch the lens.
>>>>>>>>
>>>>>>>> Thanks for your help.
>>>>>>>> *Gabriel Lapointe, M.Sc.*
>>>>>>>> Lab Manager / Microscopy Specialist
>>>>>>>> Concordia University, Biology Department
>>>>>>>> 7141 Sherbrooke St. West SP 534
>>>>>>>> Montréal QC H4B 1R6 Canada
>>>>>>>> Lab : (514) 848-2424 x5988
>>>>>>>> Office : (514) 848-2424 x3008
>>>>>>>> Fax : (514) 848-2881
>>>>>>>> Cell : (514) 278-0247
>>>>>>>> [log in to unmask]
>>>>>>>> cmac.concordia.ca
>>>>>>>> http://gabriellapointe.ca
>>>>>>
>>>>>>
>>>>>
>>>>>
>>>>>
>>>>> --
>>>>>
>>>>>
>>>>> Joel B. Sheffield, Ph.D
>>>>> Department of Biology
>>>>> Temple University
>>>>> Philadelphia, PA 19122
>>>>> Voice: 215 204 8839
>>>>> e-mail: [log in to unmask]
>>>>> URL: http://astro.temple.edu/~jbs
>>>>>
>>>>
>>>>
>>>>
>>>> --
>>>>
>>>>
>>>> Joel B. Sheffield, Ph.D
>>>> Department of Biology
>>>> Temple University
>>>> Philadelphia, PA 19122
>>>> Voice: 215 204 8839
>>>> e-mail: [log in to unmask]
>>>> URL: http://astro.temple.edu/~jbs
>>>>
>>>
>>>
>>> ****************************************************************************
>>> * Tina (Weatherby) Carvalho * [log in to unmask]
>>> *
>>> * Biological Electron Microscope Facility * (808) 956-6251
>>> *
>>> * University of Hawaii at Manoa *
>>> http://www.pbrc.hawaii.edu/bemf*
>>>
>>> ****************************************************************************
>>
>>
>> --
>> Matthew Nicholas
>> Medical Scientist Training Program Student
>> Laboratory of Arne Gennerich
>> Department of Anatomy and Structural Biology
>> Albert Einstein College of Medicine
>> Forchheimer Building, Room 628
>> 1300 Morris Park Avenue
>> Bronx, New York 10461
>> 718.430.3446
>> [log in to unmask]
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