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July 2013

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Subject:
Re: Red fluorescent proteins suitable for two-color two-photon microscopy and protein tagging
From:
Steffen Dietzel <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 2 Jul 2013 11:34:57 +0200
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A note of caution concerning the sequential imaging approach,  930->1040 
or 850->1000: Most objectives won't be color corrected in that range, so 
it is quite possible that the excitation with these two wavelengths may 
be several microns apart in z.

For testing this, I'd recommend quantum dots dried on a glass surface: 
Excitable with any wavelength and giving a bright signal, of which the 
peak is easy to determine in a z-stack. I used 665 nm emission dots, but 
others should work to.

Steffen


On 02.07.2013 10:18, Tobias Rose wrote:
(deleted)


-- 
------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
Head of light microscopy

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