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Richard,

I'm very curious to find out what your third ABRF study will be using as a "thick", cheap test specimen for confocal imaging. How does it compare to the precision of dimensions for the test specimen listed on the Argolight webpage (one of the Argolight patterns is a cylinder with about 250 nm thick fluorescent walls extending 30 um deep, 50 nm precision of features). What shape/pattern will your test specimen contain?

John Oreopoulos
Staff Scientist
Spectral Applied Research Inc.
A Division of Andor Technology
Richmond Hill, Ontario
Canada
www.spectral.ca

On 2014-03-18, at 8:46 AM, Richard Cole wrote:

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> 
> I want to thank John for pointing out ABRF's
> (http://www.abrf.org/index.cfm/group.show/LightMicroscopyResearchGroup.54.ht
> m) involvement in this very process.   This is exactly what the Light
> Microscopy research group, with the help of imagers world-wide, is trying to
> establish. We have distributed free test samples as well as detailed
> protocols in two previous studies, which tested a wide variety of instrument
> parameters.  John has provided the references for those two studies, check
> them out for more details including protocols and world-wide results. For a
> more in-depth look at PSF see: Cole, R.W., Jinadasa, T., and Brown, C.M.
> (2011) Resolution and Quality Control of Confocal Microscopy Optics. Nature
> Prot. 6 (12): 1929-1941. doi:10.1038/nprot.2011.407 
> 
> 
> 
> The current study, about to go public, will move away from the coverslip and
> provide data and metrics from where we all image, i.e. 0-100um above the
> coverslip.  We have spent over a year brain-storming, designing and testing
> a sample that will work and believe that we have achieved what we set out to
> accomplish.   I don't not believe that there is a single test specimen that
> will work for all the necessary tests. The goal was and still is, to provide
> very inexpensive test specimens and protocols along with expectable metrics
> to enable users to routinely check their instruments.  
> 
> 
> 
> Watch the list sever for more information regarding the upcoming test and
> once again free samples and protocols.   
> 
> 
> 
> Thanks to all that have participated in previous studies!
> 
> 
> 
> 
> 
> 
> 
> Richard Cole
> Research Scientist V
> Director: Advanced Light Microscopy & Image Analysis Core 
> Wadsworth Center
> 
> 
> 
> Research Assistant Professor 
> Dept. of Biomedical Sciences 
> School of Public Health State University of New York 
> 
> 
> P.O. Box 509 Albany N.Y. 12201-0509
> 518-474-7048 Phone
> 518-474-4430 Fax 
> 
> 
> 
> Email  <mailto:[log in to unmask]> [log in to unmask]
> 
> Website  <http://www.wadsworth.org/cores/alm/index.htm>
> www.wadsworth.org/cores/alm/index.htm
> 
> 
> 
> 
> 
>