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You can also release the focus lock without moving in z by holding the ‘escape’ button in and then pressing the ‘refocus’ button. I try to impress upon my users that they should never ‘refocus’ from someone else's ‘escaped’ position.

Regards,
Carol
 
Carol E. Norris, Ph.D
Facility Scientist
Flow Cytometry/Confocal Microscopy Facility
Biotechnology/Bioservices Center
University of Connecticut Unit 3149
91 N. Eagleville Rd
Storrs, CT 06269-3149

Phone (860) 486-3080
Fax (860) 486-5005

On Jun 17, 2014, at 11:46 AM, Sylvie Le Guyader <[log in to unmask]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
> 
> Another alternative for the xyz positions is to use the NIS element user log in. This way the xyz positions are saved for each user and do not show up in the other users' account.
> 
> I also tell users not to tick in the 'move stage to xy position' as this easily leads to accidentally moving the stage. Instead we found that if you double click in the x or y columns, the stage moves there anyway even if the 'move stage' box is unticked. Single click simply selects the line so that it can be edited. Accidents are less likely to happen if one needs to double click.
> 
> Med vänlig hälsning / Best regards
>  
> Sylvie
>  
> @@@@@@@@@@@@@@@@@@@@@@@@
> Sylvie Le Guyader
> Live Cell Imaging Unit Manager
> Dept of Biosciences and Nutrition
> Karolinska Institutet
> Hälsovägen 7
> Novum, G lift, floor 6
> 14157 Huddinge
> Sweden
> office: +46 (0) 8 5248 1107 
> LCI room 1: +46 (0) 8 5248 1172
> LCI room 2: +46 (0) 8 5248 3542
> mobile: +46 (0) 73 733 5008
> 
> 
>> -----Original Message-----
>> From: Confocal Microscopy List
>> [mailto:[log in to unmask]] On Behalf Of Feinstein,
>> Timothy
>> Sent: 17 June 2014 17:40
>> To: [log in to unmask]
>> Subject: Re: Limiting the z travel range on Nikon Ti
>> 
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>> 
>> Hi Shalin,
>> 
>> Using the same hardware we have also found the escape button to be quite risky
>> between users.  Of equal concern is preserving saved XYZ positions.
>> We have had to replace expensive bits (but not yet an objective, knock on
>> woodŠ) when the stage automatically tries to return to a previous user¹s XYZ
>> position that was set with a very different stage insert installed.
>> As a result we ask all users to manually lower the objectives and delete their saved
>> XYZ positions when finished.
>> 
>> All the best,
>> 
>> 
>> TF
>> 
>> Timothy Feinstein, Ph.D. | Confocal Manager
>> 333 Bostwick Ave., N.E., Grand Rapids, Michigan 49503
>> Phone: 616-234-5819 | Email: [log in to unmask]
>> 
>> 
>> 
>> 
>> 
>> 
>> 
>> On 6/17/14, 11:20 AM, "Sylvie Le Guyader" <[log in to unmask]> wrote:
>> 
>>> Hi Shalin
>>> 
>>> I assume that this happens because one user escapes the objective at
>>> the end of the imaging session and leaves the system on for the next
>>> user who then presses the reload button.
>>> Indeed I do not understand why Nikon has chosen to lock the focus wheel
>>> when the objective has been escaped so that the only way to get in
>>> focus again is to reload.
>>> 
>>> In the meantime I would suggest you do as us: tell users to
>>> systematically shut down the software and hardware (leaving only the
>>> gas lasers on) between 2 users. This way when the next user turns the
>>> system on (it only takes a minute), the objective will be automatically
>>> escaped and the focus wheel will be unlocked.
>>> 
>>> Hope that helps :)
>>> 
>>> Med vänlig hälsning / Best regards
>>> 
>>> Sylvie
>>> 
>>> @@@@@@@@@@@@@@@@@@@@@@@@
>>> Sylvie Le Guyader
>>> Live Cell Imaging Unit Manager
>>> Dept of Biosciences and Nutrition
>>> Karolinska Institutet
>>> Hälsovägen 7
>>> Novum, G lift, floor 6
>>> 14157 Huddinge
>>> Sweden
>>> office: +46 (0) 8 5248 1107
>>> LCI room 1: +46 (0) 8 5248 1172
>>> LCI room 2: +46 (0) 8 5248 3542
>>> mobile: +46 (0) 73 733 5008
>>> 
>>> 
>>>> -----Original Message-----
>>>> From: Confocal Microscopy List
>>>> [mailto:[log in to unmask]] On Behalf Of Shalin
>> Mehta
>>>> Sent: 17 June 2014 16:47
>>>> To: [log in to unmask]
>>>> Subject: Limiting the z travel range on Nikon Ti
>>>> 
>>>> *****
>>>> To join, leave or search the confocal microscopy listserv, go to:
>>>> 
>>>> http://scanmail.trustwave.com/?c=129&d=od6g0zsIvVNfDzegQ8BtCuVmNylcDld
>>>> tMr
>>>> Os9h-cGA&u=http%3a%2f%2flists%2eumn%2eedu%2fcgi-
>> bin%2fwa%3fA0%3dconfoc
>>>> alm
>>>> icroscopy
>>>> Post images on
>>>> http://scanmail.trustwave.com/?c=129&d=od6g0zsIvVNfDzegQ8BtCuVmNylcDld
>>>> tMu atpBXISA&u=http%3a%2f%2fwww%2eimgur%2ecom and include the link in
>>>> your posting.
>>>> *****
>>>> 
>>>> Hello listers,
>>>> Our lab has damaged two objectives so far by running them into stage
>>>> on our  inverted Nikon Ti by using the 'Escape' and 'Return' for Z.
>>>> 
>>>> Our old Nikon (finite tube length) has a mechanical stop on how close
>>>> the objective  can come to the stage and all old objectives are in
>>>> great shape.
>>>> 
>>>> Can we program the limits on Z travel on Nikon Ti? Asking our local
>>>> Nikon folks  suggests that the answer is No and we should avoid using
>>>> escape/return.
>>>> 
>>>> If this feature is not in the firmware, this is a request to Nikon
>>>> Japan for such a  feature.
>>>> 
>>>> Thanks
>>>> Shalin