CONFOCALMICROSCOPY Archives

January 2015

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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Subject:
From:
Avi Jacob <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Sun, 4 Jan 2015 17:23:32 +0200
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*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

I second CellProfiler.
There is a learning curve like in any program, but for non-programers like
me, it was a godsend. I had tried the turn-key ScanR analysis package, and
found CP to be superior.

I now use it even for small data-sets and I highly recommend it.
They have good support too. When you try to build a pipeline yourself
(always start with the example ones) and get stuck, you can upload what you
made together with some example images to the forum, and they will usually
help you out - and if you are lucky they will even edit your pipeline.
Sometimes takes a couple days, but still, well worth it.

Avi


--




On Sun, Jan 4, 2015 at 12:36 AM, Doug Richardson <[log in to unmask]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Have you had a look at Cell Profiler? http://www.cellprofiler.org
>
> -Doug
>
>
>
> > On Jan 3, 2015, at 5:13 PM, Grzegorz Chodaczek <[log in to unmask]>
> wrote:
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Dear confocal community,
> > I'm interested in a ready-to-use software for high content analysis of
> data
> > acquired on a Zeiss spinning disk Cell Observer with a motorized stage. I
> > was wondering whether any of you ever compared Columbus (Perkin Elmer)
> and
> > MetaXpress (Molecular Devices) or any other similar software? I would
> > appreciate any feedback about their pros and cons.
> >
> > Best regards,
> > Grzegorz
>

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