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Hi Peter,

I had hoped someone else would suggest parts because I am very curious
as well.  Right now I am using B&H ACA amplifiers for time-resolved
fluorescence measurements:

http://www.becker-hickl.com/pdf/Dbacal.pdf

These have very fast response times and good noise performance, as
well as low cost, but are sensitive to input/output loading and prone
to self-oscillation.  More annoyingly, they are AC coupled, meaning
that if you want to use them for intensity imaging, you will have to
perform more complex processing to extract the absolute intensity from
the pulse amplitude.

I would be interested to know what else is recommended as well.
Reading up on transimpedance amplifiers (and pulling a few apart), it
seems good design is sort of an art.

Mike

Mike

On Tue, Jan 27, 2015 at 4:40 AM, Peter Rupprecht
<[log in to unmask]> wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear list,
>
> I'm looking for preamplifiers for use in a resonant scanning 2P microscope, using fluorophors like GCaMP or OGB in vivo. Acquisition will be time-locked to the 80 MHz laser pulses, so the bandwidth of the PMT preamplifier should be at least 80 MHz in order not to smooth adjacent pixels too much.
>
> Right now, I'm using this model, which seems to be used by many others in the field (as far as I can see):
>
> http://www.femto.de/en/products/current-amplifiers/variable-gain-up-to-200-mhz-dhpca.html
>
> Is there another option which might be better than this standard solution for some applications? I'd be glad to hear some opinions on that.
>
> Best regards,
> Peter