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Post images on http://www.imgur.com and include the link in your posting.
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You might also want to consider Cell Profiler, which may make the task a bit easier. The software is available, and you may be able to use some of their example pipelines (such as Cell/particle counting and scoring the percentage of stained objects) with just some modifications.

Julio.
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Julio Vazquez
Fred Hutchinson Cancer Research Center
Seattle, WA 98109

http://sharedresources.fhcrc.org/core-facilities/scientific-imaging



On Jun 30, 2015, at 7:37 AM, Mike Tighe wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
> 
> would anybody know if it is possible to import a 3 or 4 color image into imageJ and have the software locate cells or objects that have signal in all or some of those channels? For example I have one channel for CD8 Tcells and I would like to have the software show me which of those CD8's are positive in one or two other channels. Rather than doing this manually.
> 
> Thanks for any help!!
> Mike