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For all of those who are not really close to this field a little excerpt from the recent literature.



Whole-Brain
Imaging with Single-Cell Resolution Using Chemical Cocktails and Computational
Analysis. http://www.ncbi.nlm.nih.gov/pubmed/24746791 (Cell) Comments: Cubic, GFP

iDISCO: A Simple, Rapid Method to Immunolabel Large Tissue Samples for Volume
Imaging. http://dx.doi.org/10.1016/j.cell.2014.10.010 (Cell) Comments: many organs,
iDISCO

A Simple Method for 3D Analysis of Immunolabeled Axonal Tracts in a Transparent
Nervous System. http://dx.doi.org/10.1016/j.celrep.2014.10.037 (Cell) Comments:
iDISCO, axonal guidance

Optimization of CLARITY for Clearing Whole-Brain and Other Intact Organs. http://eneuro.org/content/2/3/ENEURO.0022-15.2015
(ENEURO) Comments: Clarity


Many more publications on different clearing methods and the use of the Ultramicroscope system can be found at http://www.lavisionbiotec.com/ultramicroscope-literature.html



Best regards

Bernd Mueller-Zuelow

--

LaVision BioTec GmbH

Dr. Bernd Mueller-Zuelow

Area Sales Manager

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> Date: Mon, 29 Jun 2015 17:55:41 -0400
> From: [log in to unmask]
> Subject: Lightsheet z.1 vs ultramicroscope
> To: [log in to unmask]
> 
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
> 
> Dear Listers,
> 
> We are considering getting a light sheet setup for our core. I had a chance
> to test briefly the Z.1 system from Zeiss and prepare to send some samples
> to Germany to test the Ultramicroscope. Havy any of you worked with either
> of these systems? I would be very thankful for any suggestion/comment
> advice on this matter.
> 
> Our typical application is imaging of CLARITY cleared mouse brain samples.
> High resolution may be very beneficial (imaging of axonal tracks) but not
> absolutely necessary at this point. The Z.1 can work with a "CLARITY" long
> working distance objectives, but the immersion media is limited to
> non-organic solvents, so no BAAB/iDISCO, etc. Ultramicroscope as I have
> learned today, can be used with any objective, but I wonder how is
> chromatic aberration correction handled (hope to hear about that from the
> company soon).
> 
> Thank you in advance,
> Vladimir