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Hi Gary,

DPI is just dots-per-inch, so 8''x11'' (inches) at 300 dpi is an image of
2400x3300 pixels. If your camera outputs, say, 1392x1040, you have three
options, assuming your na+objective+magnification+pixel size combination is
already calibrated to capture the maximum resolution efficiently:

A. perform a mosaic of 3x3. You get a larger area of your sample.
B. perform a mosaic of 3x3, with a higher magnification (3 times, e.g.).
You get the a higher resolution image of the same area. You might have to
deal with the different depth-of-field of the different objective (e.g.
stacks+EDF).
C. scale the image up by a factor of 3 (in ImageJ/Fiji the menu item is
"Image/Scale..."). You get a "bigger" image, you comply with the
requirements, but you're going to get a blurry image, because you're
"guessing" what values are between pixels.

Hope this helps!
Thomas


On Mon, Aug 3, 2015 at 1:14 PM, Gary Laevsky <[log in to unmask]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi All,
>
> I'm trying to wrap my head around this, but it's taking way longer than it
> should, so I'm just going to expose my ineptness in this subject and ask.
>
> I need "high res" images to turn into wall art.  300 dpi is the number I've
> been given.
>
> I'm trying to make a contest out of this in the department, but I don't
> know what resolution image I have to ask for, ie how big the pixel should
> be?
>
> I'm sure the size of the ultimate "art" is important, but let's say it's
> going to be an 8' x 11' print.
>
> Little help please?  Thank you.
>
> --
> Best,
>
> Gary Laevsky, Ph.D.
> Confocal Imaging Facility Manager
> Dept. of Molecular Biology
> Washington Rd.
> Princeton University
> Princeton, New Jersey, 08544-1014
> (O) 609 258 5432
> (C) 508 507 1310
>