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I agree with John and Chris, you probably have a warped dichroic. You can
confirm using a laser pointer. Bounce the beam off your dichroic, and let
it propagate several meters (the further the better). Does the reflection
diverge asymmetrically? Does the asymmetry rotate as you rotate the
dichroic about an axis normal to its surface?

I also agree that you can buy your way out of the problem with a thicker
dichroic specified to have good flatness. This is important for both TIRF
and SIM.
On Nov 1, 2015 23:32, "Philip Nicovich" <[log in to unmask]> wrote:

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> All,
>
> I'm trying to set up a dual-camera TIRF system using two cameras and a
> dichroic splitter before the pair of matched tube lenses.  The reflected
> arm has some serious astigmatism that doesn't change with swapping cameras
> or tube lenses or playing with the alignment.  The transmitted arm is
> fine.  Swapping dichroics has a little bit of an effect, but not as much as
> I would think.  I certainly haven't found one that actually works well in
> my stash.
>
> Any tricks to confirming that the dichroic splitter is causing the
> problem?  Or what I should be looking for in a proper filter to ensure this
> doesn't happen again?
>
> Thanks,
> Rusty
>
>
>
> --
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> *Philip R Nicovich*
>
> *Research Fellow,  **ARC Centre of Excellence in Advanced Molecular
> Imaging*
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