Then, it would be better to use spectral FRET.  Measure the reference spectrum of the autofluorescence from unlabeled sample.  Use linear unmixing to remove autofluorescence. 

Sheng 


Sent from my T-Mobile 4G LTE Device


-------- Original message --------
From: "Anthony, Neil" <[log in to unmask]> 
Date:11/19/2015  3:06 PM  (GMT-06:00) 
To: [log in to unmask] 
Cc:  
Subject: Autofluorescence in PFRET 

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Hi all, I hope the science treats you well.

I was wondering if anybody's had any experience with autofluorescence when using PFRET, or PeriFRET as I like to call it.  I understand that autofluorescence is not accounted for in the algorithm?

Peri, I hope all is well with you.  Can I ask for some pointers on how think about the system if autofluorescence is the mix?

Thanks in advance for your time.

Neil

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