CONFOCALMICROSCOPY Archives

May 1995

CONFOCALMICROSCOPY@LISTS.UMN.EDU
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Subject:
Fluorescence Resonance Energy Transfer (FRET)
From:
"Peter B. Sargent" <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 17 May 1995 14:14:59 -0700
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We are interested in examining protein-protein interactions in the plane
of the membrane using FRET and wonder if anyone out there has any
experience with this technique.  The plan is to label proteins indirectly
via a Fab or IgG to which has been added a donor or acceptor.  The
classic D/A pair seems to be FITC/TRITC, but we may try Cy3/Cy5.
 
Thanks
 
Peter Sargent
UCSF

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