You asked :
 
 
>I am now engaged in studying the phagosytosis by confocal imaging with
>Fluosphere as a probe. I want to known whether Amiloride is a specific
>macropinocytosis inhibitor without any other significant effect on the
>cell function. If you know something about this chemical, please tell me
>by email or post on this board.
>
Amiloride is a sodium channel blocker that is quite effective at blocking
the epithelial Na transport, and reducing the transepithelial potential.
According to the Sigma catalog it also blocks T-type calcium channels and
Na+/H+ antiport.
I expect it will have many  effects  on cell function other than inhibiting
pinocytosis. We have used it as to disrupt embryo development.
John Cork
Calcium Imaging Facility
Department of Anatomy, LSU Medical Center
New Orleans, Louisiana 70112
Tel (504) 568 7059       Fax (504) 568 4393
e-mail  ---  [log in to unmask]