>We have a couple of users who want to use the confocal to look at calcium
>and/or pH changes on suspension cells (culture cells).  Does anyone know of
>specific tricks for trying to stabilize single/suspension cells in a
>perfusion chamber.  We would like to follow a single/small group of cells
>throughout treatment with different agents.  We have tried both charged and
>coated coverslips (silane and gelatin) without sucess.  Any and all help
>would be greatly appreciated.
 
I depend what cells they are but I would try polylysine coated coverslips. I
will not hold the cells if you are rapidly perfusing and some cells are
affected by it. If it does not work try other substrates to grow the cells
on eg. Matrigel, CellTack(sp?) collagen etc.
Good Luck
 
John Cork
Calcium Imaging Facility
Department of Anatomy, LSU Medical Center
New Orleans, Louisiana 70112
Tel (504) 568 7059       Fax (504) 568 4393
e-mail  ---  [log in to unmask]