On Wed, 10 Jul 1996, Dr. Erich Staudacher wrote:
> Hi,
> in our lab we are interested in reconstructing neurons from confocal images
> and get quantitative data on their volumes, neurite length, angles between
> neurites,.... For these purposes we are currently looking for software (Mac
> or SGI).
> 1.) Reconstruction: therefore we not only want a nice picture of a single
> stack of optical sections, but we would have to combine different stacks
> like a mosaic or puzzle to get the whole reconstruction. The stacks used
> for this stem as well from the same histological section as from adjacent
> ones.
> 2.) The measurements we would like to make (volumes, neurite length, angles
> between neurites,...) should not be done on a 2D-projection of the data,
> but in the 3D-data.
> If anyone knows one or more programs that can be used for our purposes, I
> would be glad to get the information.
> Thanks a lot,
> Erich
>
> ******************************************************************************
> Dr. Erich Staudacher
> Universitaet Leipzig
> Fakultaet fuer Biowissenschaften, Pharmazie und Psychologie
> Institut fuer Zoologie
> Talstr. 33
> D-04103 Leipzig
> phone: FRG + 0341/97 36-872
> fax: FRG + 0341/9 60 30 99
> e-mail: [log in to unmask]
> ****************************************************************************
> **
If you would be willing to consider a PC based system, MicroBrightField in
Colchester, VT USA has just the product you need:
Neurolucida. It does everything you have asked for; I know because I am
the originator of the product (it's been in existence for over 8 years,
originally with NIH support).
Neurolucida is in world-wide use, including Germany. And yes, I have an
interest in the company. Just an old university professor going commercial.
For more details, look at our web site: http:// www.microbrightfield.com/
Edmund Glaser, D. Eng.
Dept. Physiol.
Univ. Md. School. Med.
Baltimore, MD 21201 USA
Ph: (410) 706-5041
Fax: (410) 706-8341
---------- Forwarded message ----------
Date: Wed, 10 Jul 1996 07:19:22 +0100
From: Dr. Erich Staudacher <[log in to unmask]>
To: Multiple recipients of list CONFOCAL <[log in to unmask]>
Newgroups: bit.listserv.confocal
Subject: Reconstructions, measurements,...
Hi,
in our lab we are interested in reconstructing neurons from confocal images
and get quantitative data on their volumes, neurite length, angles between
neurites,.... For these purposes we are currently looking for software (Mac
or SGI).
1.) Reconstruction: therefore we not only want a nice picture of a single
stack of optical sections, but we would have to combine different stacks
like a mosaic or puzzle to get the whole reconstruction. The stacks used
for this stem as well from the same histological section as from adjacent
ones.
2.) The measurements we would like to make (volumes, neurite length, angles
between neurites,...) should not be done on a 2D-projection of the data,
but in the 3D-data.
If anyone knows one or more programs that can be used for our purposes, I
would be glad to get the information.
Thanks a lot,
Erich
******************************************************************************
Dr. Erich Staudacher
Universitaet Leipzig
Fakultaet fuer Biowissenschaften, Pharmazie und Psychologie
Institut fuer Zoologie
Talstr. 33
D-04103 Leipzig
phone: FRG + 0341/97 36-872
fax: FRG + 0341/9 60 30 99
e-mail: [log in to unmask]
****************************************************************************
**
>
Edmund Glaser, D. Eng.
Dept. Physiol.
Univ. Md. School. Med.
Baltimore, MD 21201 USA
Ph: (410) 706-5041
Fax: (410) 706-8341
|
