Hello Jon - we apologise if you feel that the documentation is
     inadequate. Here is what you do: With the MRC 1000, MPL is run from
     CoMOS - changing the scale factor (for XY) and the z-axis calibration
     in the setup menu in CoMOS will also change it when you switch to MPL.
     You can check this by doing a length command in CoMOS, switch to MPL
     and do another length command on the same object. If you really need
     to change the factors whilst in MPL, you can do this as follows: at
     the MPL> prompt do SETUP - this is a submenu. At the setup> prompt, do
     Factor or Fac and press return. This will tell you what the current XY
     factor is. To change it, do eg setup>fac 20.00 to get a scale factor
     of 20.00. Confirm this by doing setup>Fac <return> and it should say,
     the current scale factor is 20.00. The xy scale factor is F=(L x M X
     Z)/p where L is a known length in the specimen (microns), M is the
     objective lens magnification currently in use, Z is the live zoom
     factor and p is the number of pixels corresponding to this known
     length. In practice, if you do a length measurement on a stage
     micrometer and the answer is twice the actual value, then divide the
     scale factor by 2 either using CoMOS or MPL setup submenu. For the
     zfactor, get into the MPL setup submenu and do setup>zfac <return>.
     This will give you two numbers- the first is the sytem calibration
     factor (0.1 for a microscope with 100 microns per revolution i.e
     Nikon) and the second is the motor backlash setting which you should
     not change.

     I hope this helps you.

     Anna Smallcombe (Bio-Rad Micreoscopy Division)


______________________________ Reply Separator _________________________________
Subject: Bio-Rad MPL Calibration
Author:  Confocal Microscopy List <[log in to unmask]> at
Internet
Date:    12/02/97 18:31


Hello,

Can someone please let me know how to set the "scale factor" and the
"focus motor distance/revolution" for Bio-Rad MPL.  This is easy enough
to do in COMOS, but the rotten documentation provided by Bio-Rad doesn't
offer any suggestions for doing this _just_ in MPL.

Thanks,

jh