Charles Cranfield inquired,

>        Can anyone give me information as to what anti mouse, or rat,
>antibodies are availiable against T cells (both CD4 and CD8). I need
>an Ab  with a red dye attached (like rhodamine) that are excited using an argon
>ion laser. I'm also trying to keep clear of anti CD3 antibodies as they may
>activate the T cells, which is not what I want.
>

An Ab to CD2 is probably your best bet because it recognizes both subsets
of T cells but shouldn't activate them (although anti-CD2 blocks certain
cell-to-cell interactions if you're interested in these).  Any of the flow
cytometry companies (try Coulter or Becton-Dickonson) should sell an anti
mouse CD2 dierectly labelled with phycoerythrin or rhodamine.

Hope this helps,

Janine


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Janine LaSalle Ph.D.            [log in to unmask]
Instructor of Pediatrics
Genetics Division               (617)355-7577 (phone)
Children's Hospital             (617)355-7588 (FAX)
300 Longwood Ave.
Boston, MA  02115

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