CONFOCALMICROSCOPY Archives

March 1997

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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Subject:
From:
Renato Arruda Mortara <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 11 Mar 1997 08:29:08 +300
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Dear Colleagues,

May be someone out there can help me: We've a BioRad 1024 UV system
with Lasersharp 1024 version 2.1A. For some reason it refuses to
properly collect sequential Z-series, i.e., with triple labeled
samples, after selecting  the appropriate settings for sequential
collection, only one (usually FITC) out of three (+DAPI + Cy3) is
properly saved . Files xxx02.pic, xxx03.pic are also generated, but
contain no image.
Has anyone experienced similar problems ? Any ideas as to how to
solve this problem ?

Many thanks,
Renato Arruda Mortara
Univversidade Federal de Sao Paulo
ESCOLA PAULISTA DE MEDICINA
Rua Botucatu, 862 6o. andar
04023-062 Sao Paulo SP
Brasil
Email [log in to unmask]
Fax : 55 11 571-1095
Fone: 55 11 570-8306

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