Dear Listmembers,

I have been asked  about non-fluorescent dyes for counterstaining of
fixed material. I do live cell work and have never come across
this problem so far. Maybe someone else has experience in this area.


The interested person wants a brightfield counterstain for  sections
that will

1: be stable in aqueous mountant

2: not be blue! (the stain has to be used in conjunction with alcian
blue)

3: not show up on any of the fluorescent channels.

a yellow/brown stain would be ideal, but any suggestions would be
welcome.


Thank you very much in advance


Sabine
_______________________________________
Sabine Fischer
DIVISION of BIOLOGICAL SCIENCES
Institute of Cell and Molecular Biology
The University of Edinburgh
Daniel Rutherford Building
King's Buildings
Mayfield Road
Edinburgh EH9  3JH
Fax. 0131 650 5392
Tel. 0131 650 5326 or 5334
Email: [log in to unmask]