Dear List members,
sorry that the following question was asked twice. I was not aware
that it had already been send by the advice seeking person himself.
Sabine Fischer
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I have been asked about non-fluorescent dyes for counterstaining of
fixed material. I do live cell work and have never come across this
problem so far. Maybe someone else has experience in this area.
The interested person wants a brightfield counterstain for sections
that will
1: be stable in aqueous mountant
2: not be blue! (the stain has to be used in conjunction with alcian
blue)
3: not show up on any of the fluorescent channels.
a yellow/brown stain would be ideal, but any suggestions would be
welcome.
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_______________________________________
Sabine Fischer
DIVISION of BIOLOGICAL SCIENCES
Institute of Cell and Molecular Biology
The University of Edinburgh
Daniel Rutherford Building
King's Buildings
Mayfield Road
Edinburgh EH9 3JH
Fax. 0131 650 5392
Tel. 0131 650 5326 or 5334
Email: [log in to unmask]