>Many years back as a graduate student I used DAPI to image individual
>chromosomes in dividing sea urchin embryos.  There was a concentration in
>which the cell cycle timing was undistubed and you could see each
>chromosome clearly for at least three cell divisions.  At higher
>concentrations the chromosomes did not condense as well and tore themselves
>apart during cell division.  As my thesis involved microtubules and the
>spindle I never pusued the cause and effect of the problem.

Thanks for the advice

Unfortunately

a: we already use DAPI as a nuclear marker (for cell density)  and

b: it doesn't give the sharp borders that our 3D reconstruction package
needs (this part is a mess!)

As you may have seen, someone else advised bismark brown - so I'll try that

Thanks again

Jonathan Bard

%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%

                      Jonathan Bard

Anatomy Department, Edinburgh University, Edinburgh EH8 9AG, UK

Telephone (44) (0)131.650.3107    Fax: (44) (0)131.650.6545

%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%