I am interested a reply to this also. Multi-photon acquisition is
impressive in its live imaging capabilities and for the depth
it can penetrate into a sample. I haven't seen good
acquisition of double, much less triple labelled samples. We ended up
collecting GFP and Texas red with the krypton-argon laser and Hoechst with
the Ti-sapphire.

Paulette Brunner
W.M.Keck Center for Advanced Studies in Neural Signaling
University of Washington
Seattle, WA 98195-7290

(206) 685-8784
(206) 685-0619 (fax)

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On Wed, 20 Aug 1997, Harvey J. Karten, M.D. wrote:

> Steve,
>         Nice summary of the recent multi-photon workshop. You didn't mention
> anything about the feasibility to do double/triple labeling of
> fluorophores witgh a 2/3 photon system? I know that you could separate
> the emission spectra, but can you reliably generate single fluorophorer
> excitation with this system? I was much intrigued by your comment that
> you are ready to pitch the confocal in favor of the multi-photon, but
> you made not comment about any remaining virtues of the standard
> confocal. Can you comment?
>
> --
> Harvey J. Karten, M.D.
> Dept. of Neurosciences
> University of California @ San Diego
> La Jolla, CA 92093-0608
> WCBR EMail: [log in to unmask]
> Other EMail: [log in to unmask]
> Phone (Lab): 619-534-4938
> FAX (Lab): 619-534-6602
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> Retina Information System: http:/www-cajal.ucsd.edu
>