At the same multiphoton symposium Hans Gerritsen (Debye Institute, Utrecht)
showed the feasibility of doing fluorescence lifetime imaging using a
multiphoton instrument.  When commercial versions of a multiphoton
instrument become equipped with lifetime detectors detection of multiple
fluorophores could then be possible,  based on their fluorescence
liftetime.

>I am interested a reply to this also. Multi-photon acquisition is
>impressive in its live imaging capabilities and for the depth
>it can penetrate into a sample. I haven't seen good
>acquisition of double, much less triple labelled samples. We ended up
>collecting GFP and Texas red with the krypton-argon laser and Hoechst with
>the Ti-sapphire.
>
>Paulette Brunner
>W.M.Keck Center for Advanced Studies in Neural Signaling
>University of Washington
>Seattle, WA 98195-7290
>
>(206) 685-8784
>(206) 685-0619 (fax)
>
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>


 R. Howard Berg, Ph.D.
 Department of Microbiology &
        Molecular Cell Sciences
Campus Box 526041
University of Memphis
Memphis, TN, 38152-6041
E mail: [log in to unmask]
phone: 901-678-4449   fax: 901-678-4457