Dear Franco

You could try with FITC or Acridine orange (blue light excitation) but they
have high photobleaching. 
Safranin (green light excitation) is better than both FITC and Acridine orange.
Also the rhodamine fluorocroms family (rhodamine B, rhodamine 123) are
possible and they have a high resistence to photobleaching (green light
excitation).
But the best (at least in my work) has been Texas red (Sulforhodamine 101
acid chloride, absoption max.= 596, emission max= 620). It has a high
resistence to photobleaching and high intensity. You can see details in:
Martinez-Nistal, A et al. “3D reconstruction of wood fibres using confocal
microscopy” Microscopy and Analysis, January 1998, pp 19-20.

I hope this help you. 

MARTA
--------------------------------------------------
Marta A. Guervos
Servicio de Proceso de Imagenes
Universidad de Oviedo. SPAIN
e-mail: [log in to unmask]
URL: http://www10.uniovi.es
--------------------------------------------------

>Hello:
>I am studying celular walls in potatoes during frying using laser
>scanning confocal
>microscopy in its fluorescence mode ( we have lasers of 488 and 514
>nm).  For this reason I need a fluorochrome specific for vegetable walls
>
>whose excitation wavelength be in the range of the lasers we have.    Do
>
>you know any fluorochrome for staining cellular capable of being excited
>
>by any of the mentioned lasers?  I will appreciate any help or
>information you give me.  Thanks in advance for your attention .
>--
>MSc Franco Pedreschi
>PhD Student
>Pontificia Universidad Católica de Chile
>Department of Chemical Engineering and Bioprocesses
>e-mail:[log in to unmask]
>Phone:(56-2)6864264
>Fax:(56-2)6865803
>