You'll likely never get rid of the autofluor completely.  One approach that
may work if you are not terribly concerned about fluorescence intensity is
to show (hopefully bright) GFP plus minor amounts of autofluor with a BP
530/30 (the narrower the bandpass, the better), then use an LP 515 and/or
LP 580 to show GFP plus LARGE amounts of autofluor.  Put these two (three)
images side-by-side and I would believe your interpretation if handed them
to review.  The bacterial cells should be obvious unless you have a very
low mag.  Your irregular meat surface could be imaged nicely with a
water-immersible (dipping) lens.
Rob Palmer

>Dear All,
>along the same lines of the Gram +/- stains issue....I am attempting to
>image raw beef samples inoculated with GFP-Labeled bacteria with the Zeiss
>LSM 510 and have encountered autofluorescence in the beef interfering with
>the GFP. Any suggestions on filters to use which would block out the
>autofluorescence while still allowing me to visualize the beef (as a
>transmitted light image perhaps) as well as the GFP-labeled microorganisms?
>I've tried "tweaking' channels, filters, colors and am running up quite a
>bill on the scope. Thanks in advance!
>
>
>
>
>Tatiana A. lorca
>M.S./Ph.D. candidate in Food Science and Technology
>Lab 20
>FST building
>Virginia Tech
>Blacksburg VA 24061
>(540)231-8448
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