We always save the individual image files.

Susan Garfield
Laboratory of Experimental Carcinogenesis
Division of Basic Sciences
National Cancer Institute, NIH
Building 37, Room 3C28
37 Convent Drive MSC4255
Bethesda, MD  20892-4255
Phone: 301-496-5688; Fax: 301-496-0734



        -----Original Message-----
        From:   Doug Cromey [SMTP:[log in to unmask]]
        Sent:   Wednesday, July 28, 1999 11:13 AM
        To:     [log in to unmask]
        Subject:        Appropriate way to save BioRad files?

        A colleague here at the University asked me a question that I wasn't
sure
        how to answer.  He uses a BioRad 1024 confocal microscope.  We were
talking
        about saving confocal files and he told me that he only saves the
combined
        images, not the individual image files from the FITC and TRITC images.
        Since I use a Leica TCS-4D, I'm not very familiar with the BioRad
confocal.
         I told him that I wasn't sure if that was the appropriate way to save
the
        files, from the standpoint of data integrity and being able to "work
with"
        the images later.  Since I wasn't sure, I offered to ask the question on
        this Listserv.  Any feedback?

        Yours,
        Doug
        .....................................................................
        : Douglas W. Cromey, M.S.          Dept. of Cell Biology & Anatomy  :
        : Research Specialist, Principal   University of Arizona            :
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