I took this to mean that he was saving the red, green and blue channels
together into an RGB image rather than saving 2 separate grey scale (or
paletted) images.  I personally prefer the RGB method (we have the Zeiss LSM
410) for dual or triple images, to simplify bookkeeping.  Most software that
can read RGB images will let you split up the channels later if need be,
without too much effort.  The downside:it requires a little more skill with
the software.  There is no problem with data integrity that I know of.


Jeff Reece
Biomedical Engineer
Confocal Microscopy Center
National Institute for Environmental Health Sciences
P.O. Box 12233, MD F2-02
Research Triangle Park,  NC  27709
(919) 541-0311
[log in to unmask]


> ----------
> From:         Doug Cromey[SMTP:[log in to unmask]]
> Reply To:     Confocal Microscopy List
> Sent:         Wednesday, July 28, 1999 11:12 AM
> To:   [log in to unmask]
> Subject:      Appropriate way to save BioRad files?
>
> A colleague here at the University asked me a question that I wasn't sure
> how to answer.  He uses a BioRad 1024 confocal microscope.  We were
> talking
> about saving confocal files and he told me that he only saves the combined
> images, not the individual image files from the FITC and TRITC images.
> Since I use a Leica TCS-4D, I'm not very familiar with the BioRad
> confocal.
>  I told him that I wasn't sure if that was the appropriate way to save the
> files, from the standpoint of data integrity and being able to "work with"
> the images later.  Since I wasn't sure, I offered to ask the question on
> this Listserv.  Any feedback?
>
> Yours,
> Doug
> .....................................................................
> : Douglas W. Cromey, M.S.          Dept. of Cell Biology & Anatomy  :
> : Research Specialist, Principal   University of Arizona            :
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> : (FAX:  520-626-2097)           (email: [log in to unmask]):
> :...................................................................:
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