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Thank you Benjamin! The Thorlabs system hast a position sensor implemented, so I plan on using it closed-loop with an external drive voltage to keep it phase-locked to my microscope acquisition. I can then simply adjust the phase of the driving signal to match the objective movement. This should suffice to account for any delayed movement wrt the driving signal. Of course, if the temperature changes drastically during the measurement, the phase might drift, but I guess I will either have to make my own experience with this or hope that someone here has already done this and can report it. 

Best,
Nino

> Am 04.05.2021 um 22:36 schrieb Benjamin Smith <[log in to unmask]>:
> 
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> 
> I'm not in the lab, so I don't know exactly what piezo-Z we have, but it
> does drive the Olympus 20x/1.0NA dipping objective just fine with 30-50 µm
> square wave jumps at 30 Hz.  The biggest thing is to make sure the driver
> you choose has a position sensor output (ideally as an analog voltage).  As
> you allude to, objectives have a fair amount of inertia so you need the
> position feedback voltage to know when the objective actually arrives at
> the new position so you can add a corresponding delay to the scan.  This
> way, you can test your step sequence before you start imaging and record
> the actual position as a function of time.  Then take the feedback curves
> to determine how much time you'd like to wait before starting the next
> scan.  For bonus points, you could readily implement this as a closed loop
> feedback - meaning all the decisions happen without user input.
> 
> Also, as stated in Appendix D of the Thorlabs manual for the driver you
> reference, the repeatability and hysteresis of a piezo driver changes with
> temperature and age, and thus the manual also recommends using a position
> sensor to resolve these issues.
> 
> On Tue, May 4, 2021 at 12:54 PM Sebastian 'Nino' Karpf <[log in to unmask]>
> wrote:
> 
>> *****
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>> 
>> Dear list,
>> 
>> can you recommend a piezo objective scanner?
>> I want to buy a fast (~10-20Hz sinusoidal @ 200µm range) piezo scanner to
>> acquire multiphoton 3D volumes. Currently, I’m opting for the Thorlabs
>> piezo system PFM450E. Objective will be either a Nikon CFI75 25X NA 1.1 /
>> Olympus XLPLN25X NA 1.05 (or the like from Leica or Zeiss). So overall
>> rather massive/heavy objectives.
>> 
>> So my questions are:
>> - Do you have experience with fast motion (>10Hz sinusoidal for 200µm
>> range)? How is the movement? Any hysteresis?
>> - Do you prefer the Thorlabs system or a PI Pifoc (or similar system)? I’m
>> opting for the Thorlabs system due to the resonant mode at up to 120Hz for
>> 150g objectives.
>> - Can this be easily implemented into Labview/Micromanager/ICY etc.
>> software environment?
>> 
>> Any recommendations or literature references are welcome!
>> 
>> Thank you,
>> Nino
> 
> 
> 
> -- 
> Benjamin E. Smith, Ph. D.
> Imaging Specialist, Vision Science
> University of California, Berkeley
> 195 Weill Hall
> Berkeley, CA  94720-3200
> Tel  (510) 642-9712
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