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| Date: | Wed, 13 Oct 2010 16:07:11 -0400 |
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I think most people get the pixel size from an image of a stage micrometer
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Christophe Leterrier
Sent: Wednesday, October 13, 2010 3:04 PM
To: [log in to unmask]
Subject: Re: Pixel size
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If there is no other magnifying/demagnifying optics in your
microscope, the theoretical pixel size is 4.65/40=116.25 nm
Christophe
On Wed, Oct 13, 2010 at 20:43, <[log in to unmask]> wrote:
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> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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>
> Bonjour à tous,
>
> I have a tiff image, 1392 x 1040 - 4.65 um square pixels acquired with a
> camera interline Sony , 1.4 megapixel, color(7.6mm x 6.2mm array). I use
> a 40 x objective mounted on a table microscope Leica DME.
>
> Can I find the pixel size in hte image?
>
> Thanks ,
>
> Louis
>
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