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Good afternoon,
I am sorry that I am late with adding a comment, the content of which
possibly already had been mentioned (although I did not find it).
While fused silica essentially is "glassy" in its character - i.e.
optically isotropic - quartz and sapphire are crystals (trigonal) with
bi-refringence properties. There will, hence, not be a single refractive
index but there will be different refractive indices for different
orientations.
I assume that the producers of quartz or sapphire cover slips take this
into account. However, when using lenses with large NAs it will be
un-avoidable to have different parts of the light cone passing the cover
slip to experience different refractive indices.
Best wishes,
Johannes
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> Thanks to everybody for your advice. I will take all this into
> consideration.
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]]
> On Behalf Of Peter Gabriel Pitrone
> Sent: Thursday, December 22, 2011 3:00 AM
> To: [log in to unmask]
> Subject: Re: imaging through quartz or fused silica
>
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> Hello Yevgeniy,
>
> I would recommend figuring out the correct thickness of the needed
> coverslips before purchasing them... If the R.I. is significantly higher
> in quartz, which I believe it is (~1.6ish), then you need to calculate how
> thick it needs to be to match 0.1700 mm of the type of glass used in
> normal coverslips (~1.52ish).
>
> Good luck!
>
> Pete
>
> On Wed, December 21, 2011 11:47 pm, Yevgeniy Romin wrote:
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> |
> | Dear List
> |
> | Has anybody here done confocal imaging through quartz or fused silica,
> | rather then through a glass coverslip? One of our users is
> | irradiating his yeast cells using UV light through the bottom of a
> | multi well plate before doing a timelapse, but it seems that the
> | irradiation is not at
> all
> | efficient through glass. It's recommended that it be done through
> quartz
> | or fused silica. He wants to do live imaging immediately after
> | irradiation, so he does not have the time to transfer the yeast
> anywhere,
> | he has to image in whatever he irradiated in. He can have a plate
> custom
> | made (with quartz cut to glass coverslip thickness), and I was
> | wondering if anybody had any experience here with something similar.
> |
> | Thanks to all of you in advance,
> |
> | Yevgeniy Romin
> |
> |
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>
> --
> Peter Gabriel Pitrone - TechRMS
> Microscopy/Imaging Specialist
> Prof. Dr. Pavel Tomancak group
> Max Planck Institute for
> Molecular Biology and Genetics
> Pfotenhauerstr. 108
> 01307 Dresden
>
> "If a straight line fit is required, obtain only two data points." - Anon.
>
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