Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

But, it will depend upon the highest RI component of the sample.  you  
might have to go higher or lower by blending.  I've used Arnie Voie's  
mixture of 5 parts methyl salicylate:3 parts benzyl benzoate to  
obtain an RI that calculates to about 1.556.   It works well for  
dense connective tissue of bone.

Regards,

Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923  USA
(206) 616-4156
[log in to unmask]

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On Feb 6, 2008, at 7:11 AM, Mayandi Sivaguru wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Judy, try the BABB after Dent's fixative. BABB or Murray's clear  
> stands
> for 1:2 mixture of benzyl alcohol and benzyl benzoate both RI  
> around 1.5
> and available from Sigma. Let me offline should you have problem or
> success with this protocol.
> Thanks
> Shiv
>
> Mayandi Sivaguru PhD, PhD
> Microscopy Facility Manager
> Institute for Genomic Biology
> Unviersity of Illinois at Urbaba-Champaign
> Urbana 61801 IL USA
> [log in to unmask]
> http://core.igb.uiuc.edu
>
>
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> What's the best method of making a sample more transparent to reduce
>> scattering, without changing its chemistry too much? (other than  
>> methyl
>> salicylate and glycerol).
>>
>> Thanks.
>> Judy
>>
>> Judy Trogadis
>> Bio-Imaging Coordinator
>> St. Michael's Hospital, 7Queen
>> 30 Bond St.
>> Toronto, ON M5B 1W8, Canada
>> ph:  416-864-6060  x6337
>> pager: 416-685-9219
>> fax: 416-864-6043
>> [log in to unmask]
>>
>>
>>>>> Bill Miller <[log in to unmask]> 02/04/08 9:16 PM >>>
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> ultimately the lower NA with its longer working distance wins if the
>> sample is clear enough..
>>
>>
>> At 08:31 PM 2/4/2008 -0500, you wrote:
>>> Search the CONFOCAL archive at
>>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>> Hello all!
>>>
>>> I was wondering for which one penetration depth is higher: NA:1.2
>>> 60x lens or NA : 0.3 10x lens?
>>>
>>> thanks
>>> Sarah
>>