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I'm very interested in what comes out if this since we are
seriously interested in getting such a system.  The
obvious question is 'Could you see anything whan you looked
through the eyepiece?'

The most likely reason I can think of is that the disk
was slowing down more and more and finally stopped.  Which
speed Yokogawa head do you have?


                                            Guy Cox




Quoting "Puhalla, Susan" <[log in to unmask]>:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> We've purchased a new Perkin Elmer Ultraview-( with AOTF) system earlier
> this year and it was installed about two months ago. However, we have been
> experiencing some problems with it and so far, we have not been able to
> resolve them.
>
> First, as we were using it during one session, we began to see a black
> strip
> across the bottom of the image. At the same time, we began seeing lines
> from
> the pinholes even at a 1 SECOND  exposure time ( Perkin Elmer does not
> recommend exposure times below  50ms because below 50ms you will begin to
> see the pinholes spinning).
>
> Finally, during this same session on the microscope, we were suddenly
> unable
> to see any image, there was no laser light coming through at the objective,
> and the software slowed down significantly and kept freezing on us. The
> exposure time also began jumping up to 9999ms all on its own. Of course, we
> checked the obvious- like the light was being directed to the camera and
> not
> the eyepiece as well as checking to make sure that a dichroic was not in
> place. To make sure it wasn't the laser itself, we checked the 488nm,
> 568nm,
> and 647nm laser lines- with no success
>
> Has anyone experienced these types of problems? What did you do to fix it?
>
> Susan Puhalla
> Microscopy Research Assistant
> MRC Clinical Sciences Centre
> Room 5003
> Hammersmith Hospital
> DuCane Road
> London W12 ONN
> UK
> Telephone: 020 8383 8528
> Email: [log in to unmask]
> Website: http://www.csc.mrc.ac.uk/Microscopy/home.html
>


--
Associate Professor Guy Cox
Electron Microscope Unit, F09
University of Sydney NSW 2006
+61 2 9351 3176

Until 25th July:
Instituto Gulbenkian de Ciencia, PT-2780-156, Oeiras, Portugal.
+351 21 446 4638 (office) or +351 91 401 5726 (mobile)
Fax: +351 21 440 7970