Chris,
	Constancy is a bit of a trick. I don't 
know that anything would be perfectly constant. A 
popular stain is calcofluor white. This stains 
cellulose and other polysaccharides. It is uv 
excited, blue emission. All cell walls will be 
stained although there will be some variation in 
brightness.

	Hope this helps,
			Tobias

>Dear Confocal community,
>
>I have a special question regarding labeling of wood cells (spruce).
>For tomography reasons I like to label wood 
>cells to improve the autofluorescence, because 
>not all
>cell structures might give a constant signal.
>So far we have managed to visualize a wood decaying fungus that grows through
>the pits and erodes the cellwalls.
>Which dyes and fluorophores might bind to all 
>elements of wood (lignin, cellulose, 
>hemicellulose)
>to produce a constant fluorescence?
>Thanks for any helpful comments!!
>
>best wishes
>
>Chris Stührk
>
>Laboratory Wood
>Woodprotection and Biotechnology
>Empa - Material Science & Technology
>Room D 1.17
>Lerchenfeldstrasse 5
>CH-9014 St. Gallen
>
>Pho: +41 (0) 71 2747622
>Fax: +41 (0) 71 2747694
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       _      ____          __   ____   
      /  \   /          / \    /   \ \        Tobias I. Baskin
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