Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Thanks for your clarifications. Does a similar logic apply to lyophilized
samples also. Does that also change for fluorescent dyes which are mostly
in some kind of organic solvents.

-Prabhakar

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> True. If the specimen is in a cryoprotectant such as glycerol mixture
> (as Kurt suggests), OCT (as Christina suggested offline), or many other
> recipes such PEG, Dextran, Sucrose etc, then ice crystals may form at
> the lower temperature.
>
> So rule of thumb; if your specimen is basically in water and is already
> frozen then the lower temperature should not make much difference. But
> if it is still in a liquid at -20 C then it should not be stored at -80.
>
> Cheers
>
> Stephen H. Cody
> Microscopy Manager
> Central Resource for Advanced Microscopy
> Ludwig Institute for Cancer Research
> PO Box 2008 Royal Melbourne Hospital
> Victoria,      3050
> Australia
> Tel: 61 3 9341 3155    Fax: 61 3 9341 3104
> email: [log in to unmask]
> www.ludwig.edu.au/labs/confocal.html
> www.ludwig.edu.au/confocal
>
> Tip: Learn how to receive reminders about you microscope booking:
> www.ludwig.edu.au/confocal/Local/Booking_Hint.htm
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]] On
> Behalf Of Kurt Thorn
> Sent: Friday, 29 February 2008 10:44 AM
> To: [log in to unmask]
> Subject: Re: -20C samples in -80C
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> The exception to this is if your sample is in a mixture (e.g.
> glycerol/water) that is liquid at -20 but frozen at -80.  Then moving
> samples from -20 to -80 may have deleterious effects on your sample.
>
> Kurt
>
> Stephen Cody wrote:
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Dear Prabhakar,
>>
>> Your specimen will be 60 Dec C cooler!  :)
>>
>> I wouldn't have thought there would be any damage to your specimen.
> -20
>> Deg C I would think is the recommended temperature colder should be
>> fine.
>>
>> It is quite different if the recommend 4 deg C. then the specimen
> should
>> not be frozen. But once frozen I can see a problem with going to -80
> Deg
>> C.
>>
>> Cheers
>>
>> Stephen H. Cody
>> Microscopy Manager
>> Central Resource for Advanced Microscopy
>> Ludwig Institute for Cancer Research
>> PO Box 2008 Royal Melbourne Hospital
>> Victoria,      3050
>> Australia
>> Tel: 61 3 9341 3155    Fax: 61 3 9341 3104
>> email: [log in to unmask]
>> www.ludwig.edu.au/labs/confocal.html
>> www.ludwig.edu.au/confocal
>>
>> Tip: Learn how to receive reminders about you microscope booking:
>> www.ludwig.edu.au/confocal/Local/Booking_Hint.htm
>>
>> -----Original Message-----
>> From: Confocal Microscopy List [mailto:[log in to unmask]]
> On
>> Behalf Of B. Prabhakar Pandian
>> Sent: Friday, 29 February 2008 8:43 AM
>> To: [log in to unmask]
>> Subject: -20C samples in -80C
>>
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Hello:
>>
>> Can any one tell me what happens if one stores samples to be  stored
> at
>> -20C in -80C freezers.
>>
>> Thanks,
>>
>> -Prabhakar
>>
>>
>> This communication is intended only for the named recipient and may
> contain information that is confidential, legally privileged or subject
> to copyright; the Ludwig Institute for Cancer Research does not waiver
> any rights if you have received this communication in error.
>> The views expressed in this communication are those of the sender and
> do not necessarily reflect the views of the Ludwig Institute for Cancer
> Research.
>>
>>
>>
>
>
> --
> Kurt Thorn, PhD
> Director, Nikon Imaging Center
> University of California San Francisco
>
> UCSF MC 2140
> Genentech Hall Room S252
> 600 16th St.
> San Francisco, CA 94158-2517
>
> http://nic.ucsf.edu
> phone 415.514.9709
> fax   415.514.4300
>
>
> This communication is intended only for the named recipient and may
> contain information that is confidential, legally privileged or subject to
> copyright; the Ludwig Institute for Cancer Research does not waiver any
> rights if you have received this communication in error.
> The views expressed in this communication are those of the sender and do
> not necessarily reflect the views of the Ludwig Institute for Cancer
> Research.
>
>