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November 2017

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From:
Peter O'Toole <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 29 Nov 2017 13:06:01 +0000
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*****
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Hi Alby

https://www.nature.com/articles/srep02369 may be of interest for QPI in
this area.

Happy to talk more about the potential and having our first own system
installed in 2 weeks time!  It is great for prolonged time-lapse and large
or multiple fields of view, but if you are after fast dynamic studies and
concentrating on a small field of view, there are other options that may be
more suitable (e.g. Tomocube, Phasics etc)

Using the Phasefocus beta-test system, we have gone on to use it for other
applications e.g.  https://www.nature.com/articles/srep22032. The new
system is a step-improvement over the system we used for this.

Best

Pete



On 28 November 2017 at 18:47, MODEL, MICHAEL <[log in to unmask]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Quantitative phase (not phase contrast) is a very big field. For example,
> there is a book
>
> Popescu G. Quantitative Phase Imaging of Cells and Tissues. New York:
> McGraw-
> Hill; 2011. 385 p.
>
> If you specifically need quantitative phase contrast, I only know of one
> publication:
>
> Farinas J, Kneen M, Moore M, Verkman AS. Plasma membrane water permeability
> of cultured cells and epithelia measured by light microscopy with spatial
> filtering.
> J Gen Physiol 1997;110:283-296.
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]]
> On Behalf Of Alberto Diaspro
> Sent: Tuesday, November 28, 2017 9:47 AM
> To: [log in to unmask]
> Subject: quantitative phase contrast
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
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> http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%
> 3Dconfocalmicroscopy&data=01%7C01%7Cmmodel%40KENT.EDU%
> 7Cddce497762ef4f9d6ade08d5368eb0d6%7Ce5a06f4a1ec44d018f73e7dd15f2
> 6134%7C1&sdata=dVBH9Yjq3yEf7hss9L1AUIZn7eJA5YdqlWVzpgZuNKY%3D&reserved=0
> Post images on https://na01.safelinks.protection.outlook.com/?url=
> http%3A%2F%2Fwww.imgur.com&data=01%7C01%7Cmmodel%40KENT.EDU%
> 7Cddce497762ef4f9d6ade08d5368eb0d6%7Ce5a06f4a1ec44d018f73e7dd15f2
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> gg%3D&reserved=0 and include the link in your posting.
> *****
>
> Dear
> I am intersted in finding papers, including reviews, about the use of
> (quantitative) phase contrast for chromatin imaging.
> Can you help?
> Best
> Alby
>
> mix.iit.it
>



-- 
Latest Publication: Correlative super-resolution fluorescence and electron
microscopy using conventional fluorescent proteins in vacuo.
<http://www.sciencedirect.com/science/article/pii/S104784771730093X>


<http://www.linkedin.com/pub/peter-o-toole/1b/b79/444>
Dr Peter O'Toole
Director of the Bioscience Technology Facility
Head of Imaging and Cytometry
Bioscience Technology Facility
Department of Biology (Area 15)
University of York
YORK
YO10 5DD

Tel : +44 (0)1904 328722
email : [log in to unmask]
www.york.ac.uk/biology/tf

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