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Hi Christoph

I used to bring Cy3 up in degassed dH2O (with argon), aliquot in to 
eppendorf tubes and capped under a flush of argon and plunge the tube in 
to liquid nitrogen. I would then store in liquid nitrogen until 
needed.   It worked well and gave reproducibe labelling ratio's.

Best

Pete

On 18/09/2014 12:29, Christophe Leterrier wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi,
>
> A question for the people who make their fluorescent probes:
>
> I have split 1 mg Cy3-NHS into 10 x 0.1 mg using 20 µL DMSO into 10 x 2 µL.
> Now I'm trying to evaporate the DMSO but it seems quite difficult (it's
> been two hours in the Speedvac/cold trap and it's still not dried). Any
> suggestions on how to proceed (or an alternative method to DMSO)?
>
> Thanks for your help,
>
> Christophe

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