Subject: | |
From: | |
Reply To: | |
Date: | Wed, 17 Sep 2014 18:12:40 +0000 |
Content-Type: | text/plain |
Parts/Attachments: |
|
|
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****
Invitrogen's Live/Dead assay includes calcein, a dye that is only taken up by live cells and it only becomes fluorescent when it is exposed to cytoplasmic esterases. It's a green dye and I am pretty sure that you can purchase it separately and not just as part of the kit.
Doug
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
Douglas W. Cromey, M.S. - Associate Scientific Investigator
Dept. of Cellular & Molecular Medicine, University of Arizona
1501 N. Campbell Ave, Tucson, AZ 85724-5044 USA
office: AHSC 4212 email: [log in to unmask]
voice: 520-626-2824 fax: 520-626-2097
http://swehsc.pharmacy.arizona.edu/micro
Home of: "Microscopy and Imaging Resources on the WWW"
UA Microscopy Alliance - http://microscopy.arizona.edu
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Eric Shelden
Sent: Wednesday, September 17, 2014 10:49 AM
To: [log in to unmask]
Subject: General fluorescent stain for cytoplasm
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****
All:
I am looking for a way to fluorescently counter-stain the volume of cultured cells, with a result similar to what would be achieved by having them express GFP in the cytoplasm. I have seen some references to staining with eosin Y. Does anyone have other suggestions?
Thanks in advance.
Eric
|
|
|