If you want to do quantitative analysis have a look at 
http://www.sipchart.org/.
This method uses coverslides with a carefully calibrated dye doped thin 
polymerfilm to get all kinds of quantitative data from you microscope 
setup (z-resolution, intensity distribution in xy plane etc.

Peter

Maria Mazzillo wrote:
> Hello,
>
> I have taken photos using fluorescent beads and am using ImageJ to compare
> the fluorescence in the sample to the beads.  I have been looking at others
> who have presented semi-quantitative data but there is a huge variation in
> how it was presented.  I was wondering if anyone has done this kind of thing
> before and what the best way is to present the data (graph, table, etc.).
>
> Thanks,
>
> Maria Mazzillo
> Auburn University
>
>   

-- 

Peter D. Zoon
Centre for Advanced Microscopy
Section of Molecular Cytology
Swammerdam Institute for Life Sciences
Faculty of Science, University of Amsterdam		

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