***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Laurent, I can't help you with the C. elegans part (there, I have only expressed eGFP & eYFP), but in mouse & rat brain tissue we have had significant success using mNeptune2 and mCardinal. Both were comparatively stable & bright, even when using STED microscopy. (Still no comparison to FPs in the green, though.) mKate2 and Tag-RFP worked quite well, too. Sorry that is all I can contribute! Nicolai >>>>>>>>>><<<<<<<<>>>>>>>>>><<<<<<<<<<>>>>>>>>>><<<<<<<<<< Dr. Nicolai T. Urban Max Planck Florida Institute Jupiter, 33458 FL, USA -----Original Message----- From: Confocal Microscopy List <[log in to unmask]> On Behalf Of Gelman, Laurent Sent: Freitag, 24. August 2018 09:52 To: [log in to unmask] Subject: Infra-Red Fluorescent protein in C. elegans ***** To join, leave or search the confocal microscopy listserv, go to: https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.umn.edu%2Fcgi-bin%2Fwa%3FA0%3Dconfocalmicroscopy&data=02%7C01%7CNicolai.Urban%40MPFI.ORG%7C0b2b32168d2449601c7d08d609caff01%7C947b45517db44636a5fd1bdcad603ed0%7C0%7C0%7C636707164819292638&sdata=bMIeYoWfHCNG2VjPH6o5%2BSXz5FQEyVWwwnmQCbRN2h4%3D&reserved=0 Post images on https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.imgur.com&data=02%7C01%7CNicolai.Urban%40MPFI.ORG%7C0b2b32168d2449601c7d08d609caff01%7C947b45517db44636a5fd1bdcad603ed0%7C0%7C0%7C636707164819292638&sdata=b3qNbCVbElEMpbhUldQxGvfmD395PiOdS38Kl6HSyv4%3D&reserved=0 and include the link in your posting. ***** Hi everybody, Has anyone ever expressed a fluorescent protein emitting in the Far-Red in C. elegans? Many thanks in advance for your help and very best regards, Laurent. Laurent Gelman Facility for Advanced Imaging and Microscopy Head Light Microscopy Friedrich Miescher Institute for Biomedical Research Maulbeerstrasse 66 4058 basel Switzerland +41 796187369
