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Course Announcement

LIVE CELL IMAGING: FLUORESCENCE & CONFOCAL TECHNIQUES AND APPLICATIONS

26 September - 1 October, 2004
Monash Micro Imaging
Monash University, Melbourne, Australia


Website details: http://www.microimaging.monash.org/LCI.htm


COURSE CONTENT
Principles of optics
Light microscopy and image formation
Fluorescence & Confocal microscopy
Fluorescent Proteins
Fluorescence applications: FRET, FRAP, FLIM
Multiphoton Microscopy
3D and Deconvolution microscopy
TIRF

INSTRUMENTATION
Leica confocal microscopes (2 x TCSNT & 1X SP2)
Olympus FV1000 spectral confocal
PerkinELmer Ultraview (2 systems)
Nikon C1
Fluorescence microscopes with deconvolution (possibly 2 systems)
Cell chambers and incubating systems
Image analysis laboratory and software


Instructors
Ian Harper, Monash University (convener)
Alan Hibbs, Biocon
Stephen Cody, Ludwig Institute
There will also be guest lecturers, applications specialists from
vendors, and postgraduate research assistants to further support the
lectures and practical activities.


AIMS
This workshop will present an intensive and comprehensive course in Live
Cell Imaging for all students and researchers of biology requiring
functional imaging of molecules, cells, tissues and organs. A wide range
of instrumentation will be available, and extensive use will be made of
cell cultures to outline imaging modalities, experimental approaches and
biological applications. This is not a course for beginners and
participants should have some prior experience of fluorescence and
confocal imaging. The course is intended to give participants a strong
overview of imaging modalities and to provide an opportunity to learn
experimental imaging using a range of modern imaging systems.


FORMAT
The course will cover 5 days: 3pm Sunday 26 September to 1pm Friday 1
October. The program provides for approximately 12 hours of theory and
over 16 hours of practical and experimental activities.  Participants
who would like to bring specific cell lines to work on are encouraged to
contact the organizer. There will also be access to an imaging
laboratory with a range of image processing software, and participants
will be encouraged to present results of their imaging activities on the
final day.


SPONSORED BY LEICA MICROSYSTEMS, OLYMPUS AUSTRALIA, PERKINELMER,
COHERENT SCIENTIFIC, LASTEK, INVITROGEN.
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Dr Ian S. Harper, Director
Monash Micro Imaging
The Microscopy & Imaging Research Facility
School of Biomedical Sciences
Building 13C, Monash University
Clayton, VIC 3800, Australia

Email: [log in to unmask]
Tel: +61 3 9905 5635;  Fax: +61 3 9905 2733
Mobile: 0408 314168

General enquiries: [log in to unmask]
http://www.microimaging.monash.org
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