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- commercial response -

Dear  Xinyu,

Imaris determined a count of 1859.

The procedure:
- drop image file into Imaris
- create new spot component
- step through detection wizzard
- open statistics

The Imaris Spot detection/analysis component knows how to handle variable
image intensity and cell size.

Let me know if you are interested in the final image (overlay of your
original image with detected spots) or an evaluation copy of Imaris.

Regards,
Marius

Marius MESSERLI, Ph.D.
CEO Bitplane
http://www.bitplane.com

-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Xinyu Zhao
Sent: Mittwoch, 6. Februar 2008 21:29
To: [log in to unmask]
Subject: advice on counting objects

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Dear listers,

 I was wondering if anybody could give me some advice on how to count the
objects in the image as attached.  They were cross sections of inner
segments of rods and the goal is to get the total number of them. 

It seemed a easy job at the beginning.  But after I started, I realizee that
it is tricky.  The intensity variation of the objects is big and the
boundaries of the cells are not clear.  

I am not much of an image processing person.  I was wondering if anybody
could give me some advice on how to proceed.

Thank you very much.

Xinyu Zhao
Biomedical Imaging Core Lab
B110 Richards Building
School of Medicine
University of Pennsylvania
37th and Spruce Street
Philadelphia, PA 19104