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Remko,

Thank you for the prompt response to the PSF thread.
 
I have another question that is somewhat related to the topic. We need to measure diameters of small intracellular bubbles that can be less than a micron across. Ours is a regular (not superresolution) system - either widefield fluorescence, or confocal fluorescence, or (hopefully, in the near future) a Fluoview 3000 with deconvolution. Has anyone proved that accurate measurements on such a small scale are possible? What would it take? Some kind of deconvolution - 2D? 3D? Does anyone know what others have done in this direction? Thank you

Mike Model