>Dear list
>
>if one wants to avoid using DAPI (because of autofluorescence), what other
>alternatives, with as much similar properties to DAPI as possible, do
>exist?
>
>Thanks
>
>********************** PLEASE NOTE NEW E-MAIL ADDRESS ************************
>
>Stamatis Pagakis                                          [log in to unmask]
>University Laboratory of Physiology          [log in to unmask]
>University of Oxford, Parks Rd.              FAX:  +44 (0)1865 272469
>Oxford OX1 3PT,   United Kingdom             Tel:  +44 (0)1865 272551
 
Many general nucleic acid stains exist, especially if you are willing to
move to visible light as the exicitation source.  See the Molecular Probes
catalogue for detailed info, but if you must keep UV excitation, try
Hoechst 33258 or 33342, or trimethylpsoralin.
Rob Palmer
CEB/UT