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Hi Sean,
though I haven't published it, I compared a number of variants with the best
linker has been described in the 2008 paper pasted below (Mat&Methods section).
Luckily my protein was still functional when fused to GFP/YFP, and I had
designed a simple test for the protein functionality, which is in part described
in the 2000 paper (Fig. 1).
1. Chukkapalli V., I. B. Hogue, V. Boyko, W.S. Hu, and A. Ono. 2008. Interaction
between HIV-1 Gag matrix domain and phosphatidylinositol-(4,5)-bisphosphate is
essential for efficient Gag-membrane binding. J. Virol. 82(5):2405-17.
2. Boyko, V., J. Ferralli, J. Ashby, P. Schellenbaum, and M. Heinlein. 2000.
Function of microtubules in intercellular transport of plant virus RNA. Nature
Cell Biol. 2:826-832. This research paper described several
temperature-sensitive mutants of the movement protein
If you have any questions, please let me know.
Good luck,
Vitaly
301-515-7833
________________________________
From: Sean Speese <[log in to unmask]>
To: [log in to unmask]
Sent: Mon, October 4, 2010 2:07:55 PM
Subject: Flexible linkers
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This is a tad bit off topic, but I was wondering if people would be
willing to share their experience with flexible linkers used when making
fusions of your protein of interest to fluorescent proteins. What are some
sequences that seem to work well? This will likely be empirical, but still
useful information for me in deciding what linkers to use in the future.
Thanks,
Sean Speese
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