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Sripad and Jim,

Yes, the DNA barcoding approach is potentially very powerful. I'm not very
familiar with it but I see this paper, published this month in Cell:
https://www.cell.com/cell/pdf/S0092-8674(18)30904-8.pdf

Otherwise, my experience is that simplicity will aid reproducibility.
That's partly what is attractive to me about the modified-hapten approach.
It sounds like you have more patience than I do regarding complex sample
processing!

I'm curious what the utility of these various techniques will be in
applications ranging from basic research to preclinical to highly
multiplexed clinical data acquisition. All have somewhat different aims &
requirements.

Jeff



On Sat, Aug 25, 2018 at 1:57 AM, S Ram <[log in to unmask]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Jim,
> Glad to note that you are mentioning Ultivue. On the same spirit, we should
> also mention Akoya biosciences which is a spinoff of Gary Nolan's CODEX
> technology. Both use dna labeled primary Abs with slight variation in
> washing strategies. As for a direct comparison between Ultivue and Perkin
> Elmer, that is something that we are potentially interested and may pursue
> at some point in time.
>
> Jeff,
> I am very familiar with perkin elmer Opal dyes and have been using them for
> well over 2 years. There are ways you can do Ab removal without doing
> microwave treatment. However you'd need an auto stainer for this (such as a
> leica bond rx or similar device).
> Opal dyes have their challenges but its not difficult to have the right
> controls to check for tissue loss/damage. The bigger challenges are with
> spectral unmixing and the downstream image analysis pipelines.
>
> Sripad
>

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